Fluorescence intensity- and lifetime-based glucose sensing using glucose/galactose-binding protein

摘要

We review recent progress in our laboratories towards developing in vivo glucose sensors for diabetes that are based on fluorescence labelling of glucose/galactose-binding protein. Measurement strategies have included both monitoring glucose-induced changes in fluorescence resonance energy transfer and labelling with the environmentally sensitive fluorophore, badan. Measuring fluorescence lifetime rather than intensity has particular potential advantages for in vivo sensing. A prototype fiber optic-based glucose sensor using this technology is being tested.